产品说明
生化/生理作用
The nucleosomal histones can be modified through reversible acetylation by histone acetyltransferases (HATs) and deacetylases (HDACs). Acetylation and deacetylation of histone proteins alters chromosome structure and affects transcription factor access to DNA. HDAC3 has an open reading frame of 428 amino acids. HDAC3 shares 53 % amino acid identity with HDAC1 and 52 % with HDAC2. Observations indicate that class II HDACs regulate transcription by bridging the enzymatically active NCOR2-HDAC3 complex and select transcription factors. All HDACs were found to be ubiquitously expressed in immune and non-immune tissues. In human myeloid leukemia THP-1 cells, HDAC3 transfection resulted in increased size, aberrant nuclear morphology and cell cycle G2/M cell accumulation. Functional activity of the expressed HDAC3 protein was confirmed in alpha-HDAC3 antibody immunoprecipitates by a histone deacetylase assay. Study suggests the participation of HDACs in cell cycle progression and activation.
外形
Clear and colorless frozen liquid solution
制备说明
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
基本信息
| NACRES | NA.26 |
产品性质
| 生物来源 | human |
| 重组 | expressed in insect cells |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | frozen liquid |
| 分子量 | ~50 kDa |
| 包装 | pkg of 5 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | 300 μg/mL |
| 颜色 | colorless to clear |
| NCBI登记号 | NM_003883 |
| UniProt登记号 | O15379 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... HDAC3(8841) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
