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位置:首页 > 品牌 > Sigma-Aldrich > PSF-CMV-SPEC-SPECTINOMYCIN RESISTANT PLASMID

PSF-CMV-SPEC-SPECTINOMYCIN RESISTANT PLASMID

品牌
Sigma-Aldrich
货号
OGS7
规格纯度
plasmid vector for molecular cloning
参考价格
4853.18 *本价格含增值税费
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服务
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数量
-+
产品名称:
PSF-CMV-SPEC-SPECTINOMYCIN RESISTANT PLASMID
expression vector
molecular cloning vector
vector
snapfast vector
plasmid vector
cloning vector
plasmid
产品介绍:

产品说明

一般描述

The expression of proteins in mammalian cells from the CMV promoter. This vector also contains a spectinomycin resistance cassette for selection and growth in bacteria.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

应用

Cloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.

序列

To view sequence information for this product, please visit the product page

分析说明

To view the Certificate of Analysis for this product, please visit www.oxgene.com

基本信息

NACRESNA.85

产品性质

形式buffered aqueous solution
分子量size 4210 bp
复制起点pUC (500 copies)
肽切割no cleavage
启动子Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
菌种筛选spectinomycin
报告基因none
运输ambient
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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