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FLYA13

货号
CB_95091901
规格纯度
95091901, from human fibrosarcoma
参考价格
4633 *本价格含增值税费
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数量
-+
产品名称:
FLYA13
FLYA-13
FLYA
产品介绍:

产品说明

细胞系来源

Human fibrosarcoma (retroviral packaging cells)

细胞系描述

FLYA13 has been developed together with FLYRD18 (Sigma catalog number 95091902) for packaging of retroviruses, it is especially useful for in vivo gene therapy. Both cell lines have been derived from HT1080 cells (Sigma catalog number 85111505). FLYA13 has been transfected with Moloney murine leukaemia virus cores with envelope glycoproteins of amphotropic murine leukaemia virus. This cell line is able to produce human complement-resistant retroviral vectors at titres consistently higher than 1x10,000,000 i.u./ml. Addition of 4μg/ml blasticidin S and 10μg/ml phleomycin into the media may enhance the packaging function. No replication competent virus has been detected in this cell line.

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y,
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

培养基

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:20, i.e., seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 ℃.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

产品性质

生物来源human fibrosarcoma
生长模式Adherent
核型Not specified
形态学Epithelial-like
产品Not specified
受体Not specified
technique(s)cell culture | mammalian: suitable
相关疾病cancer
运输dry ice

Sigma-Aldrich

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