产品说明
一般描述
Consanguineous: No
细胞系描述
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA图谱分析
A: 6802 3001
Cw: 1701
B: 4201
HLA DRA: 102
HLA DRB1: 302
HLA DRB3: 101
HLA DQAP: 4.2
HLA DQA1: 401
HLA DQB1: 402
HLA DPA1: 0301 0202
HLA DPB1: 0101 0402
血清学分析
HLA A: 68 30
HLA A ief: 68.1 30.3
HLA C: 2
HLA B: 42
HLA B ief: 42
Bw4/bw6: 6
HLA Dw: RSH
HLA DR: 18
HLA Dw2: 24
HLA DR52: 52
HLA DQ: 4
HLA DP: 1 4
培养基
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
传代培养常规
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
产品性质
生物来源 | human blood |
种族 | South African Zulu |
性别 | male |
生长模式 | Suspension |
核型 | Not specified |
形态学 | Lymphoblastoid |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
Sigma-Aldrich