产品说明
一般描述
The Csf3r (colony stimulating factor 3 receptor (granulocyte)) gene encodes a prototypical mobilizing cytokine.
应用
Granulocyte Colony-Stimulating Factor from mouse has been used in cytokine therapy.
生化/生理作用
G-CSF is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.
Granulocyte colony-stimulating factor (G-CSF) is a lineage-restricted hematopoietic growth factor, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors. G-CSF3,4 is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and mature granulocytes, and promotes differentiation of certain myeloid leukemic cells. Natural G-CSF is a glycoprotein of 177 amino acids and a molecular weight of 19 kDa.5 Human and murine G-CSF have about 75% homology and show biological cross-reactivity.
外形
Lyophilized from a 0.2 μm filtered solution in 10 mM citric acid, 0.01% TWEEN® 20, pH 4.0, containing 50 μg bovine serum albumin per 1 μg of cytokine.
分析说明
The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
法律信息
TWEEN is a registered trademark of Croda International PLC
基本信息
| MDL编号 | MFCD00166475 |
| NACRES | NA.77 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 重组 | expressed in E. coli |
| 测定 | >97% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 效能 | 10-60 pg/mL ED50 |
| 质量 | endotoxin tested |
| 分子量 | 19 kDa (179 amino acids including N-terminal methionine) |
| 包装 | pkg of 5 and 25 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | ≤0.01 EU/μg |
| UniProt登记号 | P09920 |
| 储存温度 | −20℃ |
| Gene Information | mouse ... Csf3(12985), Csf3r(12986) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H319 |
| 预防措施声明 | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| 危险分类 | Eye Irrit. 2 |
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
