BICR 18

产品说明

细胞系来源

Larynx squamous cell carcinoma (lymph node metastasis)

细胞系描述

Adherent keratinocyte cell line derived from a lymph node metastasis squamous cell carcinoma (SSC) of the larynx. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 using 0.25% trypsin/EDTA; 8% CO₂; 37 C. Suggested seeding density 4 x 10,000 cells/cm². A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line, howeve

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

产品性质