产品说明
一般描述
Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.
应用
Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.
组分
Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl
基本信息
NACRES | NA.25 |
产品性质
质量水平 | 100 |
形式 | liquid (2x concentrate) |
异质活性 | DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich