PhosphoDetect Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb

产品说明

一般描述

Recognizes c-Raf phosphorylated at Tyr³⁴⁰ and Tyr³⁴¹ in HEK293 cells overexpressing c-Raf.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr^340/341.
This PhosphoDetect Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb is validated for use in WB, ICC for the detection of c-Raf (pTyr³⁴⁰, Tyr³⁴¹).
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
Human

包装

10 tests in Plastic ampoule

应用

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

警告

Toxicity: Standard Handling (A)

外形

In phosphate buffer (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
HEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.

其他说明

The immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Yan, F., and Polk, D.B. 2001. Cancer Res.61, 963.
Yip-Schneider, M.T., et al. 2000. Biochem. J.351, 151.
Mason, C.S. et al. 1999. EMBO J.18, 2137.
Bonner, T.I. et al. 1985. Mol. Cell. Biol.5, 1400.
Moelling, K. et al. 1984. Nature312, 558.
Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA80, 4218.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息