产品说明
一般描述
Monoclonal Anti-ASPP2 (mouse IgG1 isotype) is derived from the hybridoma DX 54.10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice. Apoptosis-stimulating protein of p53 (ASPP) family of proteins contains three members, ASPP1, ASPP2 and iASPP, which interact via their C-terminal region with p53 and modulate its activity.
特异性
Monoclonal Anti-ASPP2 recognizes human and mouse ASPP2. The epitope recognized by the antibody resides within amino acids 691-1128 of human ASPP2.
免疫原
fragment of human ASPP2 (amino acids 691-1128).
应用
Western blot analysis of Hek293 or 3T3-L1 adipocyte cell lysates was performed using monoclonal mouse anti-ASPP2 as the primary.
Mouse Monoclonal Anti-ASPP2 has been used for immunofluorescence analysis. The antibody can also be used for immunocytochemistry, immunoprecipitation and microarray applications.
生化/生理作用
ASPP2 is a tumor suppressor that activates p53. ASPP2 also regulates Par-3 which subsequently modulates the proliferation and polarity of neural progenitor cells during CNS development. Furthermore, ASPP2 interacts with active Ras to induce p53 mediated cell death. ASPP2 is known to function as a biomarker for invasive pituitary adenomas.
Apoptosis-stimulating protein of p53 (ASPP2) enhances the ability of p53 to induce apoptosis ASPP1 and ASPP2 stimulates the transactivation function of p63 and p73 on promoters of different genes such as BCL2 associated X, apoptosis regulator (Bax), p53-inducible gene 3 (PIG3) and p53 upregulated modulator of apoptosis (PUMA), but not mouse double minute 2 homolog (Mdm2) or p21WAF-1/CIP1.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD08705575 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | DX 54.10, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~175 kDa |
| species reactivity | mouse, human |
| 浓度 | ~1.5 mg/mL |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable microarray: suitable western blot: 0.5-1 μg/mL using total cell extract of human osteogenic sarcoma |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q13625 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... TP53BP2(7159) mouse ... Trp53bp2(209456) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
