产品说明
一般描述
Perilipin 2 (PLIN2) is a 50 kDa major protein belonging to perilipin family. It is present on lipid droplets found in the cytoplasm of variety of cells as hepatocytes, adipocytes, muscle cells, mammary epithelial cells, fibroblasts, endothelial cells and macrophages. PLIN2 gene, spanning 14 kb, with eight exons, is located on human chromosome 9p22.1.
Monoclonal Anti-ADFP/PLIN2 (mouse IgG1 isotype) is derived from the hybridoma ADFP-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide.
特异性
Monoclonal Anti-ADFP/PLIN2 recognizes human ADFP/PLIN2.
免疫原
a synthetic peptide corresponding to a sequence at the C-terminus of human PLIN2, conjugated to KLH.
应用
Monoclonal Anti-ADFP / PLIN2 antibody produced in mouse may be used in immunoblotting and immunofluorescence.
生化/生理作用
Perilipin 2 (PLIN2) plays a vital role in uptake and storage of lipids as triacylglycerols in lipid droplets in skeletal muscle. PLIN2 is also involved in the regulation of lipid metabolism. High levels of PLIN2 is beneficial for the prevention of insulin resistance. Overexpression of PLIN2 is associated with clear cell renal cell carcinoma (CC-RCC).
PLIN2 may serve as a biomarker of lipid accumulation in diverse cell types and diseases.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | ADFP-5, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~48 kDa |
| species reactivity | human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using human HepG2 or SH-SY5Y cells western blot: 2-4 μg/mL using whole extracts of HEK-293T cells overexpressing human ADFP |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q99541 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PLIN2(123) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
