产品说明
一般描述
Jumonji domain-containing protein 2D (JMJD2D) is also called Lysine-specific demethylase 4D and JmjC domain-containing histone demethylation protein 3D. JMJD2D is a histone demethylase that specifically demethylates both di- and trimethylated H3 ′Lys-9′, but not monomethylated histone H3 ′Lys-9′ residues. JMJD2D also does not demethylate histone H3 ′Lys-4′, H3 ′Lys-27′, H3 ′Lys-36′ or H4 ′Lys-20′.
特异性
Other homologies: Bovine, Porcine, Canine, and Rhesus Macaque (93% sequence homology), Mouse (87% sequence homology), Rat (80% sequence homology).
免疫原
KLH-conjugated linear peptide corresponding to human JMJD2D.
应用
Detect Jumonji domain-containing protein (JMJD) using this rabbit polyclonal antibody, Anti-JMJD2D Antibody validated for use in western blotting, IHC & IP.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected JMJD2D in human testis and liver tissues.
Immunoprecipitation Analyis: 20 µg from a representative lot detected JMJD2D in 500 µg of HeLa cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
质量
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected JMJD2D in 10 µg of HeLa cell lysate
目标描述
~58 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | chimpanzee (based on 100% sequence homology) |
technique(s) | immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI登记号 | NP_060509 |
UniProt登记号 | Q6B0I6 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich