产品说明
一般描述
Calcium-independent phospholipase A2 (iPLA2) belongs to the group six of phospholipase A2 (PLA2) enzymes. The protein is encoded by the gene mapped to human chromosome 22q13.1. iPLA2 is an intracellular enzyme. It is present both in the cytosol and in membrane fractions. The encoded protein contains 752 amino acids and has a mass of 85kDa. It is characterized with a eight ankyrin repeats and a catalytic domain.
应用
Anti-Phospholipase A2 (iPLA2) antibody produced in rabbit has been used in western blotting.
生化/生理作用
Calcium-independent phospholipase A2 (iPLA2) in the absence of Ca2+, catalyzes the hydrolysis of the Sn-2 ester bond in phospholipids to produce free fatty acids and lysophospholipids. iPLA2 might play an essential role in activating the receptor-mediated signaling pathway. Additionally, it also yields precursors for bioactive compounds such as eicosanoid and platelet-activating factor. The encoded protein performs housekeeping role in phospholipid remodeling.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00803786 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~85 kDa (and ~95 kDa human iPLA2 and ~95 kDa mouse and rat iPLA2.) |
| species reactivity | human, mouse, rat |
| 浓度 | ~1.5 mg/mL |
| technique(s) | western blot: 1.5-3.0 μg/mL using using extracts of HEK-293T cells overexpressing human iPLA2, RAW264 cell lysates and rat pancreas extracts (S1 fraction). |
| UniProt登记号 | O60733 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PLA2G6(8398) mouse ... pla2g6(53357) rat ... pla2g6(360426) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
