Anti-IRS1 Antibody

产品说明

一般描述

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

特异性

Recognizes IRS1, Mr 160-185kDa..

免疫原

Carboxy-terminal 14 amino acid peptide ([C]YASINFQKQPEDRQ) of rat liver IRS1

应用

Anti-IRS1 Antibody is an antibody against IRS1 for use in IP & WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

质量

Routinely evaluated by Western Blot on NIH3T3 cell lysate.

目标描述

165 kDa

外形

ImmunoAffinity Purified
Purified Rabbit IgG in buffer containing 0.2 M Trisglycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, 5 mg/mL BSA, and 0.05% sodium azide.

储存及稳定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 Cell Lysate. Use 20 µg per lane for minigels.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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