产品说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
特异性
Broad species cross-reactivity expected due to sequence homology.
Recognizes C-Terminal region of Histone H3, Mr 17 kDa
免疫原
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
应用
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.
Use Anti-Histone H3 Antibody, CT, pan (rabbit polyclonal antibody) validated in ChIP, WB to detect Histone H3 also known as H3, Histone H3, H3 histone family member T, histone 3 H3, histone cluster 3 H3.
质量
Routinely evaluated by immunoblot on acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
目标描述
Approx. 17 kDa
外形
Unpurified
Rabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human, Saccharomyces cerevisiae, yeast, mouse, chicken, rat |
manufacturer/tradename | Upstate® |
technique(s) | ChIP: suitable western blot: suitable |
NCBI登记号 | NM_002107.3 |
UniProt登记号 | Q16695 |
运输 | dry ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich