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位置:首页 > 品牌 > Sigma-Aldrich > Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody

Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody

品牌
Sigma-Aldrich
货号
07-888
规格纯度
Upstate®, from rabbit
参考价格
5854.74 *本价格含增值税费
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数量
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产品名称:
Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody
40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline-rich), proline-rich Akt substrate, 40 kDa
产品介绍:

产品说明

一般描述

PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.

特异性

Recognizes phosphorylated PRAS40 (Thr246)), Mr 40 kDa.

免疫原

Peptide corresponding to amino acid region encompassing the human phospho-PRAS40 (Thr246).

应用

Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody is an antibody against phospho-PRAS40 (Thr246) for use in WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.

质量

Routinely evaluated by Western Blot on PDGF stimulated NIH3T3 cell lysates.

Western Blot Analysis:
A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.

目标描述

Approx. 40 kDa

外形

Format: Purified
Affinity purified
Purified rabbit polyclonal IgG in buffer containing PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide and 50% glycerol.

分析说明

Control
PDGF stimulated NIH3T3 cells.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
species reactivityhuman, mouse
manufacturer/tradenameUpstate®
technique(s)western blot: suitable
同位素/亚型IgG
NCBI登记号NP_001092102
UniProt登记号Q96B36
运输dry ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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