产品说明
一般描述
This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression.
特异性
Recognizes the catalytic subunit of Protein Phosphatase 6, MW ~33kDa.
免疫原
The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Ala-Val-Pro-Asp-Ser-Glu-Arg-Val-Ile- Pro-Pro-Arg-Thr-Thr-Thr-Pro-Tyr-COOH.
应用
Detect PP6C using this Anti-PP6C Antibody validated for use in WB, IP, IH(P).
Research Category
Signaling
Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-20 µg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP6 Polyclonal Antibody:
Human Testis
PP6 staining of round spermatids in normal human
testis, tissue pretreated with citrate buffer, pH 6.0.
Antibody was diluted 1:100, using IHC-Select
detection with HRP-DAB.
Research Sub Category
Kinases & Phosphatases
质量
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP6C on 10 μg of Jurkat lysates.
目标描述
Recognizes the catalytic subunit of Protein Phosphatase 6 (PP6), MW ~33kDa
联系
Replaces: AB4091
外形
Purified rabbit polyclonal IgG in buffer containing PBS with 0.08% sodium azide.
Format: Purified
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
Jurkat Cell Lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | bovine, rat, human, mouse |
technique(s) | immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_002721.3 |
UniProt登记号 | O00743 |
运输 | wet ice |
Gene Information | human ... PPP6C(5537) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich