产品说明
一般描述
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.
应用
BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.
注意
在2-8℃下可稳定至少一年。该基底具有光敏性。
其他说明
Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.
法律信息
BCIP is a registered trademark of sigma-aldrich Co. LLC
基本信息
NACRES | NA.83 |
产品性质
质量水平 | 100 |
形式 | liquid |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich