产品说明
一般描述
Human recombinant C−terminal histidine tagged caspase 8 is expressed as the truncated pro-caspase 8 (amino acids 213−496). It is a fully active protein consisting of 18 kDa and 12.4 kDa subunits; the 12.4 kDa subunit contains the histidine tag.
包装
10 μg in poly bottle
生化/生理作用
Caspase 8 is at the top of the hierarchy of the caspase cascade and is considered an initiator caspase. It exists in the cell as an inactive 55 kDa proenzyme. It is converted to the active form, consisting of 18 kDa and 11 kDa subunits, upon its recruitment to the cytoplasmic domain of activated death receptors such as CD95 or TNFR via the adapter protein FADD. The activation of the proenzyme is triggered by the protein′s aggregation, which leads to auto- or transprocessing. Caspase 8 activates downstream caspases (caspases 3, 6, and 7) that cleave key cellular substrates and lead to apoptotic death of the cells.
单位定义
One unit will cleave 1.0 nmol of Ac-Ile-Glu-Thr-Asp-pNA per min at pH 7.4 at 25 ℃.
外形
Solution in 10% sucrose containing 20 mM Tris-HCl, 150 mM imidazole-HCl, pH 8.0, 20 mM 2-mercaptoethanol, 500 mM NaCl, 2.5 mM EDTA, and 0.1% CHAPS.
基本信息
| MDL编号 | MFCD01865996 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli (C-terminal histidine tagged) |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | buffered aqueous solution |
| specific activity | ≥1,000 units/mg protein |
| 分子量 | ~30 kDa |
| UniProt登记号 | Q14790 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... CASP8(841) |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H317 - H319 - H360D |
| 预防措施声明 | P202 - P261 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| 危险分类 | Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1 |
| 储存分类代码 | 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
