Anti-CENP-E antibody produced in rabbit

产品说明

一般描述

Centromere-associated protein-E (CENP-E, 312 kDa) is a member of the kinesin superfamily of microtubule motor proteins and is an integral part of kinetochore corona fibers that link centromere to the spindle microtubules. CENP-E localizes to the kinetochore throughout all phases of mitotic chromosome movement from early prometaphase through anaphase A.

免疫原

synthetic peptide corresponding to amino acids 2545-2563 located near the C-terminus of human CENP-E conjugated to KLH.

应用

Anti-CENP-E antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunocytochemistry.

包装

200 μL in glass insert

生化/生理作用

Centromere-associated protein-E (CENP-E) plays an important role in attachment of kinetochores to spindle microtubules in the alignment of chromosomes and is an essential component of mitotic checkpoint signaling cascade. It functions as a motor in the initial chromosome movement at the mitotic midzone. CENP-E is associated with minus end-directed microtubule motor activity suggesting that CENP-E might be responsible for poleward kinetochore movements in prometaphase and anaphase A. Suppression of CENP-E synthesis by antisense CENP-E yields chromosomes that are chronically mono-oriented with flattened bipolar spindles and generates spindle poles fragments. Depletion of CENP-E leads to profound checkpoint activation and long-term mitotic arrest. CENP-E has been implicated as a binding partner for the mitotic checkpoint kinase BubR1 during mitosis.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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