产品说明
包装
25 μg in glass bottle
生化/生理作用
When cells receive apoptotic stimuli, such as activation of the TNFα/Fas cell surface receptor, caspase 8 activation, Bid processing and its translocation to the mitochondria ensue. As a result mitochondria release cytochrome c which then binds to Apaf-1, the mammalian Ced-4 homologue, together with dATP. The resultant complex recruits caspase 9 leading to its activation. It then cleaves downstream caspases such as caspase 3, 6, and 7, initiating the caspase cascade. Caspase 9 exhibits basal activity. It can be further activated via interaction of its N-terminal prodomain with the activator protein Apaf-1 in the presence of cytochrome c and dATP. Caspases have been implicated in many disorders including cancer, inflammatory disease, neurodegenerative diseases, stroke and myocardial infarction.
单位定义
One unit will cleave 1.0 nmol of Ac-Leu-Glu-His-Asp-AFC per hr at 25 ℃ at pH 6.5.
外形
Solution in 10% sucrose containing 50 mM HEPES, pH 7.5, 5 mM DTT, 0.1% CHAPS, 1 mM EDTA, 50 mM NaCl.
基本信息
| 酶学委员会(EC)编号 | 3.4.22.62 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD02687006 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli (C-terminal histidine-tagged) |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | buffered aqueous solution |
| specific activity | >2,000 units/mg protein |
| 分子量 | N-terminal prodomain plus the large subunit. 36 kDa (caspase 9 expressed as a C-terminal histidine-tagged protein appears as a two-subunit protein) small subunit 13 kDa (subunit containing the histidine tag) |
| UniProt登记号 | P55211 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... CASP9(842) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
