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Anti-Cre Recombinase Antibody, clone 2D8

品牌
Sigma-Aldrich
货号
MAB3120
规格纯度
ascites fluid, clone 2D8, Chemicon®
参考价格
7365.52 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Cre Recombinase Antibody, clone 2D8
产品介绍:

产品说明

一般描述

Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. This antibody can be used to determine the success of gene manipulations in transgenic animals.

特异性

Cre recombinase. The epitope is located between aa 77 and 343.

免疫原

Cre recombinase (aa 77 to 343) fusion protein

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.

Immunofluorescence:
A previous lot of this antibody was used on IF.

Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions must be determined by the end user.
Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.
Research Category
Epigenetics & Nuclear Function

目标描述

38 kDa

外形

Ascites fluid. Contains no preservative
Unpurified

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.

分析说明

Control
HeLa Nuclear Extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆2D8, monoclonal
species reactivity (predicted by homology)all
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG1κ
UniProt登记号P06956
运输dry ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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