您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > 肠激酶 来源于猪肠

Enterokinase from porcine intestine

肠激酶 来源于猪肠

品牌
Sigma-Aldrich
CAS
9014-74-8
货号
E0885
规格纯度
lyophilized powder, ≥100 units/mg protein
参考价格
13356.4 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
9014-74-8
Enterokinase from porcine intestine
Enteropeptidase
肠激酶 来源于猪肠
肠肽酶
产品介绍:

产品说明

应用

Enterokinase from porcine intestine has been used in a study to investigate complementary DNA cloning and sequencing of rat enteropeptidase. Enterokinase from porcine intestine has also been used to learn more about the insulinotropic region of the gastric inhibitory polypeptide.
The enzyme from Sigma has been used to activate zymogens in order to detect trypsin activity. The study to investigated the structural and evolutionary consequences of unpaired cysteines in trypsinogen. The product has been used to measure trypsin while studying the effect of pesticide induced alterations in gene expression in the lobster, Homarus americanus The enzyme from Sigma has been used to develop a novel assay for measuring levels of lipid-free apoA-I in the presence of lipid-bound apoA-I. Enteropeptidase can specifically cleave human lipid-free apoA-I but not its lipid-bound form resulting in an N-terminal fragment of 22 kDa. It has also been used in a study to examine the effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin.

生化/生理作用

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

单位定义

在 pH 5.6 和 25℃ 下,一单位每分钟可将胰蛋白酶原转化为 1.0nmol 的胰蛋白酶。

外形

含有磷酸钠缓冲盐的冻干粉

基本信息

酶学委员会(EC)编号3.4.21.9 ( BRENDA | IUBMB )
EC 号232-761-1
MDL编号MFCD00131020
eCl@ss32160410
NACRESNA.54

产品性质

形式lyophilized powder
质量水平200
specific activity≥100 units/mg protein
分子量 150 kDa
纯化方式chromatography
组成Protein, ≥20% Lowry
异质活性aminopeptidase ≤1.5%
trypsin ≤1%, free
储存温度−20℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

Sigma-Aldrich

推荐产品