您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Monoclonal Anti-hFBXW7 小鼠抗

Anti-hFBXW7 antibody , Mouse monoclonal

Monoclonal Anti-hFBXW7 小鼠抗

品牌
Sigma-Aldrich
货号
F2055
规格纯度
clone FB407, purified from hybridoma cell culture
参考价格
6557.59 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-hFBXW7 antibody , Mouse monoclonal
Anti-SEL-10 (C. elegance)
Anti-ARCHIPELAGO (Drosophila)
Anti-FBW7
Anti-F-box and WD40 domain protein 7
Anti-Cdc4 (S. serevisia)
Monoclonal Anti-hFBXW7 小鼠抗
产品介绍:

产品说明

一般描述

FBXW7 is a member of F-box protein family, which acts as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). The proteins have a bipartite structure. The shared F-box motif links F-box protein to Skp1 and the core complex, whereas divergent protein-protein interaction motifs selectively bind their cognate substrates. There are three FBXW7 isoforms α, β and γ that share 10 out of 11 exons but they differ in their subcellular localization as well as in substrate recognition activity.
Monoclonal Anti-hFBXW7 (mouse IgG2a isotype) is derived from the hybridoma FB407 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino-acids of human hFBXW7 isoforms α β and γ. FBXW7 is located on human chromosome 4q31.

特异性

The antibody recognizes human, mouse, and rat hFBXW7.

免疫原

synthetic peptide corresponding to amino-acids 238-250 of human hFBXW7 isoforms α, β, and γ.

应用

Anti-hFBXW7 antibody, Mouse monoclonal has been used in:

  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry

生化/生理作用

F-box proteins is associated with various signalling pathways, such as nutrient sensing in yeast,conserved developmental pathways in plants and animals. These proteins mediate recognition of phosphorylated targets including Cyclin E, Myc, c-Jun, and Notch, leading to their ubiquitination and degradation. Furthermore, it was found mutated in breast, endometrial, ovarian, and colon cancer, and finally results in genomic instability. Similar to the inactivation mode of other known tumor suppressors, the SV40 large T antigen binds FBXW7 without detectible effects on its stability, and thus acts as an inhibitor of FBXW7 activity.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD09265423
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆FB407, monoclonal
形式buffered aqueous solution
分子量antigen ~60 kDa
species reactivityrat, human, mouse
浓度~2 mg/mL
technique(s)immunocytochemistry: 10-20 μg/mL using human A431 or HeLa cells
indirect ELISA: suitable
western blot: 5-10 μg/mL using human A431 cell extracts
同位素/亚型IgG2a
UniProt登记号Q969H0
运输dry ice
储存温度−20℃
Gene Informationhuman ... FBXW7(55294)
mouse ... Fbxw7(50754)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

推荐产品