产品说明

一般描述

CD31 is a single chain integral membrane glycoprotein member of the immunoglobulin superfamily of cell adhesion molecules with molecular mass of 130-140kDa. It has six Ig-like loops in the extracellular domain, a trans-membrane domain including a relatively long cytoplasmic domain. It is expressed abundantly on platelets, myeloid cells, B lymphocytes, certain T-lymphocyte cell subsets, bone marrow precursor cells, NK cells and localized to the intracellular junctions in monolayers of cultured endothelial cells.
Monoclonal Anti-CD31 (PECAM-1) is derived from the WM-59 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

特异性

识别 CD31 (PECAM-1、endoCAM、gpIIa、hec7)、人细胞表面整合膜糖蛋白。该抗体可增加 TGF-β1在 U937 细胞中诱导的同型聚集率，其与血小板的结合在洗涤后增强。
第 5 次研讨会：代码P025

免疫原

人细胞系 RC-2A，最初来源于髓系白血病细胞。

应用

Monoclonal Anti-CD31 (PECAM-1)-FITC antibody has been used in

• DAB based-immunohistochemistry
• direct immunofluorescent staining
• fluorescence-activated cell sorting (FACS)
• flow cytometry

Monoclonal Anti-CD31 (PECAM-1)-FITC antibody is suitable for immunostaining of HEPC-CB2 and HEPC-CB.2 cell Lines (hematopoietic cells) and flow cytometry to evaluate the percentage of contaminating endothelia cells in characterization and decidualization of endometrial stromal cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

生化/生理作用

CD31 is also known as PECAM-1 (Platelet endothelial cell adhesion molecule-1) binds to glycosaminoglycans (GAG). Endothelial CD31 is phosphorylated on tyrosine and serine residues. CD31 is involved in some of the interactive events taking place during cardiovascular development inflammation, thrombosis, wound healing and angiogenesis.
CD31 performs in homophilic and heterophilic cell-cell adhesion and cell signalling activities. It plays a major role in the transmigration of monocytes, neutrophils and NK cells between the endothelial cell junctions into the subendothelial matrix.

目标描述

人体CD31 (PECAM-1) 在血小板、内皮细胞、骨髓细胞、B 淋巴细胞和某些 T 淋巴细胞亚群上表达。CD31 (PECAM-1) 在嗜同性和嗜异性细胞间粘附和细胞信号传导活动中起作用。

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

制备说明

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

产品性质

质量水平	200
生物来源	mouse
偶联物	FITC conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	WM-59, monoclonal
形式	buffered aqueous solution
分子量	antigen 130-140 kDa
species reactivity	human
储存条件	protect from light
technique(s)	flow cytometry: 10 μL using 1 × 106 cells
同位素/亚型	IgG1
UniProt登记号	P16284
运输	wet ice
储存温度	2-8℃
Gene Information	human ... PECAM1(5175)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)