产品说明
一般描述
Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.
应用
Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry).
生化/生理作用
Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine.
分析说明
Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.
基本信息
| MDL编号 | MFCD01867037 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in baculovirus infected Sf21 cells |
| 测定 | >97% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 效能 | 0.03-0.1 μg per mL ED50 (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells) |
| 分子量 | 55 kDa (glycosylated form) |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| UniProt登记号 | P25446 |
| 储存温度 | −20℃ |
| Gene Information | mouse ... Fas(14102) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
