产品说明
一般描述
TrkA is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.
特异性
Unconjugated version Cat.No.06-574 is predicted to react with Rat based on immunogen design. The unconjugated part number was also shown to react with Mouse.
Antibody recognizes TrkA protein.
免疫原
Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.
Epitope: Extracellular domain
应用
Research Sub Category
Neurotransmitters & Receptors
Neurodegenerative Diseases
Research Category
Neuroscience
Detect TrkA using this Milli-Mark Anti-TrkA-FITC Antibody validated for use in FC.
质量
Evaluated by flow cytometry using HeLa cells.
目标描述
TrkA exists as two isoforms: 87 kDa and 88 kDa
外形
Purified rabbit polyclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Protein A purified
储存及稳定性
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
分析说明
Control
HeLa cells
法律信息
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
偶联物 | FITC conjugate |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human |
manufacturer/tradename | Milli-Mark® |
technique(s) | flow cytometry: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NP_067600 |
UniProt登记号 | P35739 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich