产品说明

一般描述

JumpStart^™ REDTaq^® ReadyMix^™PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The Master mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.

应用

Jumpstart^™ REDTaq^® ReadyMix^™反应混合物可用于直接提取DNA并进行PCR扩增。还用于：

• 多重定量PCR
• 甲基化特异性PCR(MSPCR)
• 总RNA合成cDNA的PCR扩增

特点和优势

• JumpStart^™ REDTaq^® DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
• REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
• Inert red dye allows for easy verification that reagent has been mixed properly
• Other loading dyes are not required. Aliquots may be directly loaded onto an agarose gel after the reaction

包装

默认的反应体积为50 μL

20RXN包装成1 X 500 μL
100RXN包装成1 X 2.5 mL
800RXN包装成1 X 20 mL

单位定义

在74℃下，一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。

其他说明

想查看JumpStart REDTaq和ReadyMix产品的更多详细信息，请访问 www.sigma-aldrich.com/hotstart。
对于典型的PCR反应，将25mL JumpStart^™ REDTaq^® ReadyMix^™反应混合物与25mL混合物(含有模板DNA、引物和水)混合。可根据需要改变反应体积。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：US 8,404,464和US 7,972,828。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可。
凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权(如，5′ Nuclease Process专利权)。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
JumpStart is a trademark of sigma-aldrich Co. LLC
REDTaq is a registered trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC

基本信息

产品性质