您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-phospho-p62 (Ser403) Antibody, clone 4F6

Anti-phospho-p62 (Ser403) Antibody, clone 4F6

品牌
Sigma-Aldrich
货号
MABC186-I
规格纯度
clone 4F6, from rat
参考价格
6190.47 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-phospho-p62 (Ser403) Antibody, clone 4F6
Ubiquitin-binding protein p62, p62, EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
产品介绍:

产品说明

一般描述

Sequestosome-1 (UniProt: Q13501; also known as EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62) is encoded by the SQSTM1 (also known as ORCA, OSIL) gene (Gene ID 8878) in human Sequestosome is a multi-functional co-interacting protein with a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chains and regulates the activation of the nuclear factor kappa-B (NF-kappaB) signaling. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 (TRAF6) to mediate the activation of NF- B in response to upstream signals. It also serves as an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. Defects in sequestosome-1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and is characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Mutations in SQSTM1 gene can lead to frontotemporal dementia and/or amyotrophic lateral sclerosis 3. Frontotemporal dementia is characterized by frontal and temporal lobe atrophy associated with neuronal loss, gliosis, and dementia. Amyotrophic lateral sclerosis is characterized by the death of motor neurons in the brain, brainstem, and spinal cord, resulting in fatal paralysis.

特异性

Clone 4F6 recognizes p62/SQSTM1 phosphorylated at Ser403 in human and mouse.

免疫原

KLH-conjugated linear peptide corresponding to a sequence surrounding pSer403 of human phospho p62/SQSTM1.

应用

Western Blotting Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Western Blot application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).

Immunohistochemistry Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Immunohistochemistry application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).






Detect p62/SQSTM1 (Ser403) using this rat monoclonal Anti-phospho-p62 (Ser403) antibody, clone 4F6, Cat. No. MABC186-I. Validated for use in Immunohistochemistry and Western Blotting.

质量

Evaluated by Western Blotting in Neuro2a cells.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected p62/SQSTM1 (Ser403) in Neuro2a cells in which GFP fused human p62 (G-p62) was stably transfected and treated with MG132 vs. an untreated sample.

目标描述

~60 kDa observed; 46.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆4F6, monoclonal
species reactivityhuman, mouse
technique(s)immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_003891.1
UniProt登记号Q13501
运输ambient
Gene Informationhuman ... SQSTM1(8878)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品