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位置:首页 > 品牌 > Sigma-Aldrich > Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12

品牌
Sigma-Aldrich
货号
04-1570-I
规格纯度
culture supernatant, clone 4E12, from rat
参考价格
5688.25 *本价格含增值税费
促销
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数量
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产品名称:
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
产品介绍:

产品说明

一般描述

RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues ′Ser-2′ and ′Ser-5′ of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at ′Ser-7′ of the heptapepdtide repeat. This antibody recognizes the ′Ser-7′ CTD residue of RPB1.

特异性

Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242
This antibody recognizes RNA polymerase II subunit B1 phosphorylated at Ser7 of the C-terminal domain (CTD).

免疫原

Epitope: Phosphorylated Ser7 of the C-terminal domain (CTD)
Ovalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1 phosphorylated at Ser7 of the C-terminal domain (CTD).

应用

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12 is a highly specific rat monoclonal antibody & that targets RNA Polymerase & has been tested in western blotting.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780-1782.).
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242

质量

Evaluated by Western Blotting in untreated and lambda phosphatase treated NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-7) in 10 μg of untreated NIH/3T3 cell lysate.

目标描述

~220 kDa observed

联系

Replaces: 04-1570

外形

Unpurified
Rat monoclonal IgG1κ with 0.05% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源rat
抗体形式culture supernatant
antibody product typeprimary antibodies
克隆4E12, monoclonal
species reactivitymouse
species reactivity (predicted by homology)human (based on 100% sequence homology)
technique(s)ChIP: suitable
ELISA: suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_000928
UniProt登记号P24928
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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