产品说明

应用

Invertase Glycoprotein Standard has been used:

• to generate N-linked glycan library
• as a negative control to study the binding of lectins to high mannose structures
• for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis

The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.
用于生产糖果食品和人造蜂蜜。

生化/生理作用

转化酶将蔗糖水解成葡萄糖和果糖，产生无色产物，与产生有色产物的酸水解不同。

其他说明

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.

基本信息

酶学委员会(EC)编号	3.2.1.26 ( BRENDA | IUBMB )
EC 号	232-615-7
MDL编号	MFCD00131410
NACRES	NA.32

产品性质

质量水平	200
生物来源	Saccharomyces cerevisiae
等级	for proteomics
产品线	BioReagent
测定	≥0.5 mg/vial protein basis (E1%/280)
形式	lyophilized powder
分子量	60 kDa
储存温度	2-8℃

安全信息

象形图
警示用语：	Danger
危险声明	H334
预防措施声明	P261 - P284 - P501
危险分类	Resp. Sens. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 3
个人防护装备	Eyeshields, Gloves, type N95 (US)