产品说明
生化/生理作用
MNK2 (MAP kinase-interacting kinase 2) contains a conserved C-terminal ERK-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain. MNK2 binds tightly to the growth factor-regulated MAP kinases, ERK1 and ERK2. ERK and p38 phosphorylate MNK2, which stimulates its in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF-4E).A yeast two-hybrid screen showed the MNK2 protein interacted with the ligand-binding domain of estrogen receptor beta (ERbeta).
外形
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
基本信息
NACRES | NA.32 |
产品性质
质量水平 | 200 |
重组 | expressed in baculovirus infected Sf9 cells |
产品线 | PRECISIO® Kinase |
测定 | ≥70% (SDS-PAGE) |
形式 | buffered aqueous glycerol solution |
specific activity | 81-109 nmol/min·mg |
分子量 | ~74 kDa |
UniProt登记号 | Q9HBH9 |
运输 | dry ice |
储存温度 | −70℃ |
Gene Information | human ... MKNK2(2872) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich