产品说明
一般描述
Monocyte chemoattractant protein-1 (MCP-1) or monocyte chemotactic and activating factor (MCAF) is part of the C-C chemokine family. The gene encoding this protein is localized on human chromosome 17q12.
应用
Monocyte chemotactic protein-1 (MCP-1) has been used to study its effects on the motility of lymphoblastoid cell line NC–NC cells.
生化/生理作用
The biological activity is measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A. The ED<SUB>50</SUB> for this effect is typically 5-30 ng/mL.
Measured by its ability to chemoattract 2-day cultured human monocytes, Matsushima, K., et al. (1989) J. Exp. Med. 169:1485, the ED<SUB>50</SUB> for this effect is typically 5-20 ng/mL.
Monocyte chemoattractant protein-1 (MCP-1) triggers the activation of monocytes by triggering degranulation as well as respiratory burst activity in monocytes. It facilitates monocytes in inhibiting or enhancing the growth of specific brain tumor cell lines. MCP-1 chemoattracts monocytes, macrophages, T cells and natural killer cells. The protein is overexpressed in atherosclerosis, idiopathic pulmonary fibrosis, rheumatoid arthritis and delayed hypersensitivity reactions. It is also implicated in Kawasaki disease.
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
基本信息
| MDL编号 | MFCD00131621 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥97% (SDS-PAGE and N-terminal analysis) |
| 形式 | lyophilized powder |
| 效能 | 5-60 ng/mL EC50 |
| 分子量 | 8.7 kDa |
| 包装 | pkg of 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| UniProt登记号 | P13500 |
| 储存温度 | −20℃ |
| Gene Information | human ... CCL2(6347) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
