Monoclonal Anti-MEKK4 antibody produced in mouse

产品说明

一般描述

Monoclonal Anti-MEKK4 (mouse IgG2b isotype) is derived from the hybridoma MEKK4-338 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human MEKK4. MAP kinase 4 (MEKK4 also called MAP3K4, is the human homolog of the S. cerevisiae Ssk2 and Ssk22 MAPKKKs. The protein has 1607 amino acids with a protein kinase catalytic domain at the C-terminus that is 98, 42, 36 and 41 percent identical to the kinase domains of mouse MEKK4, Serine/threonine-protein kinase proteins (SSK22 - Ssk2, Ssk22) and S. pombe Wik1, respectively. A non-kinase domain that has a regulatory function is located at the N-terminal part of the protein. The protein is expressed mainly in heart, placenta, skeletal muscle and pancreas.

特异性

Monoclonal Anti-MEKK4 antibody is specific for human, rat and mouse MEKK4 (approx. 182 kDa).

免疫原

synthetic peptide corresponding to amino acids 1081-1095 of human MEKK4, conjugated to KLH.

应用

Monoclonal Anti-MEKK4 antibody produced in mouse has been used in:

• western blotting
• ELISA
• immunocytochemistry

生化/生理作用

MAP kinase 4 (MEKK4) -null embryos developed highly penetrant neural tube defects with exencephaly and spina bifida. During neural tube development, it regulates MAP kinase 4 (MKK4) activity and apoptotic cell death.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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