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Anti-Senataxin (OY11) Antibody

品牌
Sigma-Aldrich
货号
ABN421
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5520.38 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-Senataxin (OY11) Antibody
Probable helicase senataxin, Senataxin (OY11), Amyotrophic lateral sclerosis 4 protein, SEN1 homolog, Senataxin
产品介绍:

产品说明

一般描述

Probable helicase senataxin (UniProt Q7Z333; also known as Amyotrophic lateral sclerosis 4 protein, SEN1 homolog) is encoded by the SETX (also known as ALS4, AOA2, SCAR1, bA479K20.2) gene (Gene ID 23064) in human. Senataxin is implicated in both genome and RNA transcriptome homeostasis. Senataxin contains a helicase domain for mediating RNA interaction and an N-terminal domain for critical protein interactions. Senataxin is reported to colocalize with DNA repair proteins, including the DNA damage response protein 53BP1, at distinct nuclear foci in S/G2-phase human cells, and the number of these foci increases in response to impaired DNA replication or DNA damage. Senataxin is shown to resolve RNA/DNA hybrids (R loops) that arise during transcription, which can lead to genome instability unless they are resolved efficiently.

免疫原

A mixture of four KLH-conjugated linear peptides corresponding to sequences from both N- and C-terminal halves of human senataxin.

应用

This Anti-Senataxin (OY11) Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Senataxin .
Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated Senataxin from HeLa, U2OS, and HEK293 cell lysates (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin and siRNA-mediated Senataxin downregulation in HEK293 cells (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flag-tagged Senataxin in anti-Flag immunoprecipitates from transfected HEK293 cells expressing Flag-tagged Senataxin (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin in HEK293 cell lysates (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

质量

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Senataxin in 10 µg of HUVEC lysate.

目标描述

~300 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human
technique(s)immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号NP_055861
UniProt登记号Q7Z333
运输wet ice
Gene Informationhuman ... SETX(23064)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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