您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > 含有Taq DNA聚合酶的PCR核心试剂盒

PCR Core kit with Taq DNA polymerase

含有Taq DNA聚合酶的PCR核心试剂盒

品牌
Sigma-Aldrich
货号
CORET
规格纯度
PCR components in separate tubes to allow for optimization
参考价格
4530.77 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
PCR Core kit with Taq DNA polymerase
含有Taq DNA聚合酶的PCR核心试剂盒
产品介绍:

产品说明

一般描述

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. This enzyme is in a recombinant form, expressed in E. coli. Taq DNA polymerase enzyme can withstand high temperatures up to 95 ℃ without significant loss of activity. Taq DNA Polymerase is a magnesium ion-dependent enzyme. Taq DNA Polymerase possesses 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity.

应用

CORET PCR kit has been used for the amplification of genomic DNA during the study of genetic diversity in various species of Manilkara zapota.
PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl2 will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg2+ concentrations and the cycling parameters.

数量

试剂盒中含有足够用于250单位Taq DNA聚合酶的试剂。

其他说明

以下试剂必需但并未提供在试剂盒内:

  • 矿物油,M 8662(可选)
  • 热循环仪
  • 引物
  • 待扩增的DNA
  • 氯仿,C 7559(可选)

法律信息

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

基本信息

eCl@ss32161000
NACRESNA.55

产品性质

质量水平200
形式liquid
用途 sufficient for 250 reactions
特点dNTPs included
hotstart: no
technique(s)PCR: suitable
颜色 colorless
input purified DNA
运输wet ice
储存温度−20℃

组份列表

组份可单独销售

货号组份
P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1 mL/vial
P231710X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 1 mL/vial
M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 1 mL/vial
W1754Water, PCR Reagent 3 vial(s)
D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品