产品说明
一般描述
Diacylglycerol/diglyceride (DAG) is a glycerolipid, that is located at the plasma membrane. It is made up of two fatty acid chains that are covalently attached to a single glycerol molecule with the help of an ester linkage.
应用
18:1 DG has been used as a diacyl-glycerol source in diacylglycerol O-acyltransferase 1 (DGAT1) assay. It has also been used as substrate to assess compounds as potential inhibitors of DGAT-1 in DGAT-1 enzyme assay. It is suitable to use in lipid-protein overlay screen assay with the recombinant protein His-AtROP6.
包装
5 mL PTFE Vial with Screw Cap (800811O-10mg)
5 mL PTFE Vial with Screw Cap (800811O-200mg)
5 mL PTFE Vial with Screw Cap (800811O-25mg)
5 mL PTFE Vial with Screw Cap (800811O-500mg)
储存及稳定性
Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20℃. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.
其他说明
Effective concentration:
Most biological responses saturate at 20 to 250 μM sn-1,2-dioctanoylglycerol. Only sn-1,2 isomers appear to be active.
Delivery to cells:
Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.
Precaution: Since short chain Diacylglycerols mimic effects of the tumor-promoting phorbol diesters in a number of biological systems, extra care should be employed in their handling. Treatment of solutions, vessels and other articles with 1N NaOH before washing or discarding will destroy diacylglycerols.
基本信息
经验(实验)分子式 | C39H72O5 |
分子量 | 620.99 |
NACRES | NA.25 |
产品性质
测定 | >99% (TLC) |
包装 | pkg of 1 × 10 mg (with screw cap (800811O-10mg)) pkg of 2 × 100 mg (with screw cap (800811O-200mg)) pkg of 5 × 100 mg (with screw cap 800811O-500mg) pkg of 1 × 25 mg (with screw cap (800811O-25mg)) |
manufacturer/tradename | Avanti Polar Lipids 800811O |
运输 | dry ice |
储存温度 | −20℃ |
InChI | 1S/C39H72O5/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-31-33-38(41)43-36-37(35-40)44-39(42)34-32-30-28-26-24-22-20-18-16-14-12-10-8-6-4-2/h17-20,37,40H,3-16,21-36H2,1-2H3/b19-17+,20-18-/t37-/m0/s1 |
InChI key | AFSHUZFNMVJNKX-CSWDEDFYSA-N |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich