产品说明
一般描述
Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
应用
A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography.
Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides.
包装
50 units in glass bottle
其他说明
Insolubilized
单位定义
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 ℃ (titrimetric assay).
外形
Suspension in approx. 10 mM acetic acid, pH 3.2
基本信息
| 酶学委员会(EC)编号 | 3.4.21.4 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00082094 |
| eCl@ss | 42020142 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 生物来源 | bovine pancreas (trypsin) |
| 形式 | buffered aqueous suspension |
| 浓度 | ≥15 units/mL (packed gel) |
| 标记范围 | ≥15 units per mL packed gel |
| 基质 | cross-linked beaded agarose |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H315 - H317 - H319 - H335 |
| 预防措施声明 | P280 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 个人防护装备 | Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter |
Sigma-Aldrich
m.cnreagent.com
