产品说明
一般描述
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
应用
An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.
单位定义
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 ℃ (titrimetric assay).
外形
Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative
制备说明
TPCK-treated
基本信息
| MDL编号 | MFCD00082094 |
| eCl@ss | 42020142 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 生物来源 | bovine pancreas (trypsin) |
| 形式 | buffered aqueous suspension |
| 浓度 | ≥15 units/mL (packed gel) |
| 标记范围 | ≥15 units per mL (packed gel) |
| 基质 | beaded agarose |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H317 |
| 预防措施声明 | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| 危险分类 | Skin Sens. 1 |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
