产品说明
一般描述
Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.
应用
Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.
Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.
生化/生理作用
Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.
单位定义
One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 ℃.
外形
Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).
基本信息
| 酶学委员会(EC)编号 | 5.99.1.2 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD03458765 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 测定 | ≥2 units/μL |
| 形式 | buffered aqueous glycerol solution |
| 分子量 | 100 kDa |
| 浓度 | ≥2 units/μL |
| UniProt登记号 | P11387 |
| 储存温度 | −70℃ |
| Gene Information | human ... TOP1(7150) |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H360D |
| 预防措施声明 | P201 - P308 + P313 |
| 危险分类 | Repr. 1B |
| 储存分类代码 | 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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