产品说明
一般描述
Tubulin is one of the main components of the cytoskeleton. It is a heterodimeric structure consisting of interlocking alpha and beta chains. Like other cytoskeleton proteins, tubulin undergoes dynamic changes depending on the cellular context, which are mediated by binding and release of GDP/GTP. Tubulin proteins may also undergo post-translational modifications, and are essential to cellular transport and mitotic processes.
免疫原
Native chicken brain microtubules.
应用
Research Category
Cell Structure
This Anti-α-Tubulin Antibody, clone DM1A is validated for use in Western Blotting, ICC, IHC for the detection of α-Tubulin.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected α-tubulin in NIH/3T3, A431, and HeLa cells.
Immunohistochemistry Analysis: A 1:10,000 dilution from a representative lot detected α-tubulin in normal human brain tissue, human brain cancer tissue, normal rat hippocampus tissue and normal rat cerebellum tissue.
Research Sub Category
Cytoskeleton
质量
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.01 µg/mL of this antibody detected α-tubulin in 10 µg of HeLa cell lysate.
目标描述
~60 kDa observed. Uniprot describes a molecular weight at ~46 kDA, but can be higher due to post-translational modification. This protein has been observed at ~55 kDa in some cell lysates (Saugstad, J. A., et al. (2002). J Neurochem. 80(6):980-988.).
联系
Replaces: 04-1117
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa cell lysate.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | DM1A, monoclonal |
species reactivity | human, mouse |
species reactivity (predicted by homology) | chicken (based on 100% sequence homology) |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1κ |
UniProt登记号 | P02552 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich