Anti-ETV4 Antibody, clone 1A2G3

产品说明

一般描述

ETS translocation variant 4 protein, alternatively known as Adenovirus E1A enhancer-binding protein E1A-F, or Polyomavirus enhancer activator 3 homolog (PEA3), is encoded by the human gene named ETVA/E1AF/PEA3. ETV4 is a protein that is a transcriptional activator that was first recognized as such because it allowed for the expression of the adenovirus E1A gene upon infection. Today ETV4 has been shown to be active in a wide variety of biological processes from stem cell differentiation, to mammary gland and duct morphogenesis to motor neuron and axon guidance. ETV4 activity is regulated via the ERK/MAP signaling and a complex interplay of post-translation modifications such as sumoylation and ubiquitination. In cancers, ETV4 activates HIF and its subsequent proliferation genes and overexpression in prostate cells leads to oncogenesis. EMD-Millipore’s Anti-ETV4 mouse monoclonal antibody has been tested in western blot against K562 cell lysates as well as recombinantly expressed ETV4 protein.

免疫原

Purified recombinant fragment of human ETV4 expressed in E. Coli.

应用

Detect ETS translocation variant 4 protein using this mouse monoclonal antibody, Anti-ETV4 Antibody & clone 1A2G3 validated for use in western blotting.

质量

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ETV4 in K562 cell lysate.

Optimal working dilutions must be determined by end user.

目标描述

~54 kDa observed. Uncharacterized bands may appear in some lysate(s).

外形

Unpurified

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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