产品说明
一般描述
Wild-type p53 is present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novel marker for malignancy.
免疫原
Mouse VLM tumor cells.
应用
Anti-p53 (wild type) Antibody, clone PAb1620 is a Mouse Monoclonal Antibody for detection of p53 (wild type) also known as Cellular tumor antigen p53, Tumor suppressor p53 & has been validated in WB & IHC.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected p53 in normal human brain tissue.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p53 in IP (Lu, X., et al. (1992). Cell. 70(1):153-161.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p53 in dorsal skin samples from animal trunks (Kramata, P., et al. (2005). Cancer Res. 65(9):3577-3585.) and in certain human cancer tissues (Cordon-Cardo, C., et al, (1991). 51(23 Pt 1):6372-6380.).
Research Category
Epigenetics & Nuclear Function
质量
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected p53 in mouse brain tissue lysate.
目标描述
~53 kDa observed
外形
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Mouse brain tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | Pab1620, monoclonal |
species reactivity | mouse, human |
technique(s) | immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG2aκ |
NCBI登记号 | NP_001120705 |
UniProt登记号 | P02340 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich