产品说明
一般描述
Maspin (mammary serine protease inhibitor), also known as SERPINB5 (serpin family B member 5), was first identified in 1994. It is an epithelial-specific protein that belongs to the serine protease inhibitor superfamily known as serpin. It shows major expression in human mammary epithelial cells.
Recombinant human Maspin is a 42.2kDa non-glycosylated protein containing 375 amino acid residues.
生化/生理作用
Maspin (mammary serine protease inhibitor) functions as a tumor suppressor in various types of cancer, such as breast cancer, prostate cancer etc. Its expression in cancer is thought to be controlled by DNA methylation, and in breast cancer and glioma its expression is silenced by abnormal methylation. In tissues of pulmonary adenocarcinoma (AC) patients, the mRNA expression of maspin is significantly increased, and hence, maspin mRNA expression might have potential as a prognosis marker in AC. Study in two MIBC (muscle invasive bladder cancer) cell lines (T24 and 5637) shows that overexpression of maspin can increase cisplatin chemosensitivity. In MIBC patients receiving cisplatin based neoadjuvant chemotherapy, the expression of maspin correlates with the prognosis.
Recombinant human Maspin is a 42.2kDa non-glycosylated protein containing 375 amino acid residues.
序列
MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP
外形
Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥98% (HPLC) ≥98% (SDS-PAGE) |
| 形式 | lyophilized |
| 分子量 | 42.2 kDa |
| 包装 | pkg of 20 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | <0.1 EU/μg endotoxin, tested |
| 颜色 | white to off-white |
| UniProt登记号 | P36952 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... SERPINB5(5268) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
