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Monoclonal Anti-phospho-RNA polymerase II CTD (pSer2) antibody produced in rat

品牌
Sigma-Aldrich
货号
SAB4200637
规格纯度
clone 3E7C7, purified from hybridoma cell culture
参考价格
6732.28 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Monoclonal Anti-phospho-RNA polymerase II CTD (pSer2) antibody produced in rat
hRPB220
RpIILS
POLRA
RPB1
RPBh1
polymerase (RNA) II (DNA directed) polypeptide A 220kDa (POLR2A)
hsRPB1
POLR2
RPO2
RPOL2
产品介绍:

产品说明

一般描述

Monoclonal Anti-phospho-RNA polymerase II CTD (pSer2 ) (rat IgG2a isotype) is derived from the hybridoma 3E7C7 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide containing phospho-Ser2 of human RNA Polymerase II CTD repeat. RNA polymerase II subunit A (RNAPII), also known as POLR2A, is the largest subunit of RNA polymerase II. The POLR2A gene is mapped to human chromosome 17p13.1. This polymerase contains a carboxy-terminal domain (CTD) composed of heptapeptide repeats. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase.

特异性

Monoclonal Anti-RNA polymerase II CTD (pSer2) recognizes specifically phospho-Ser2 RNAPII but not phosphor-Ser5 or phosphor-Ser7.

免疫原

synthetic peptide containing phospho-Ser2 of human RNA Polymerase II CTD repeat.

应用

Monoclonal Anti-phospho-RNA polymerase II CTD (pSer2) antibody produced in rat may be used in:

  • immunoblotting
  • immunofluorescence
  • flow cytometry

生化/生理作用

RNA polymerase II subunit A (RNAPII) is responsible for synthesizing messenger RNA in eukaryotes. The carboxy-terminal domain (CTD) is essential for polymerase activity. The CTD mediates the coupling of transcription and processing of the nascent RNA. It also plays roles in transcription, elongation and termination. Also, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. CTD is being dynamically modified during the transcription cycle. The phosphorylation of serine is needed for RNA capping and splicing factors recruitment.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For extended storage, freeze at -20 0C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not usedwithin 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.41

产品性质

质量水平200
生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆3E7C7, monoclonal
分子量 ~250 kDa
species reactivityrat, mouse, human, canine, monkey
浓度~1 mg/mL
technique(s)flow cytometry: 10 μg/test using using HeLa cells.
immunoblotting: 0.25-0.5 μg/mL using whole extracts of HeLa cells.
immunofluorescence: 4-8 μg/mL using using A549 cells
同位素/亚型IgG2a
UniProt登记号P24928
运输dry ice
储存温度−20℃
Gene Informationhuman ... POLR2A(5430)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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