α-L-Fucosidase Assay Kit

产品说明

一般描述

α-L-Fucosidase (AFU) is an enzyme coded by the FUCA1 gene in humans and catalyzes the breakdown of L-Fucose. A genetic deficiency in this enzyme results in a neurovisceral storage disease, fucosidosis, which is characterized by the accumulation of fucose. Low serum activity of fucosidase has also been linked to ovarian caricinoma. Elevated fucosidase serum activity has been observed in patients with diabetes, hyperthyroidism, cirrhosis, and hepatitis. Increased activity has been associated with lung, breast, stomach, ovary, uterus, and liver carcinomas.

特点和优势

• High sensitivity and wide linear range. Linear detection range (10 μL sample): 1 to 100 U/L.
• Simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the quantitative determination of α-L-Fucosidase activity in plasma, serum, tissue, cell lysate, etc.

原理

The non-radioactive, colorimetric α-L-Fucosidase Assay Kit is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

产品性质

安全信息

象形图
警示用语：	Warning
危险声明	H319
预防措施声明	P264 - P280 - P305 + P351 + P338 - P337 + P313
危险分类	Eye Irrit. 2
储存分类代码	10 - Combustible liquids
WGK	WGK 2