Acid Phosphatase Assay Kit

产品说明

一般描述

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

特点和优势

• Linear detection range (20 μL sample): 0.05 to 60 U/L for a 30 minute reaction.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the determination of acid phosphatase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)

原理

The non-radioactive, colorimetric Acid Phosphatase Assay Kit is based onthe cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H302 + H332 - H314 - H341 - H373 - H411
预防措施声明	P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
危险分类	Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3