Alkaline Phosphatase Assay Kit

产品说明

一般描述

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.

特点和优势

• High sensitivity and wide linear range. The detection limit is 2 U/L, linear up to 800 U/L.
• Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc.

原理

The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction isdirectly proportional to the enzyme activity.

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H290 - H302 + H332 - H314 - H341 - H373 - H411
预防措施声明	P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
危险分类	Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3