产品说明
特异性
Specifically reacts with the hCL(VI). This is a purified mouse monoclonal antibody to human type VI collagen. VI-26 does not react with denatured or reduced type VI collagen, although this antibody does recognize the the triple helix consisting of alpha1(VI), alpha 2(VI) alpha3(gamma). The epitope has not been mapped and the monoclonal is know not to react with collagens I, II, III, IV or V in native assays.
应用
Research Category
Cell Structure
Anti-Collagen Type VI Antibody, clone VI-26 detects level of Collagen Type VI & has been published & validated for use in ELISA, IH(P).
Research Sub Category
ECM Proteins
Immunohistochemistry on acetone fixed paraffin-embedded tissues. Formalin fixation is not recommended. Antigen recovery is treatment with 0.04% trypsin in 0.01M CaCl(2) 0.05M Tris-HCL pH 7.6, 37°C for 10 minutes before the non-specific peroxidase block.
EIA
Optimal working dilutions must be determined by end user.
外形
Protein A purified
Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.
Format: Purified
储存及稳定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Testis, connective tissue
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | VI-26, monoclonal |
species reactivity | human, rabbit |
should not react with | rat |
manufacturer/tradename | Chemicon® |
availability | not available in Japan |
technique(s) | ELISA: suitable immunohistochemistry: suitable (paraffin) |
同位素/亚型 | IgG1κ |
NCBI登记号 | NM_001848.3 |
UniProt登记号 | P12109 |
运输 | dry ice |
Gene Information | human ... COL6A1(1291) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich