产品说明
一般描述
Monoclonal Anti-Desmoglein 2 (DSG2) (mouse IgG1 isotype) is derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells. Desmogleins are a family of cadherin proteins. There are four distinct desmoglein genes (DSG1-DSG4), each differentially expressed depending upon the type of tissue and the state of differentiation. Desmoglein-2 (DSG2) is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis and heart.
免疫原
derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells.
应用
Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse has been used in immunoblotting and immunofluorescence.
生化/生理作用
Desmogleins play an important role in the formation of desmosomes, cell to-cell adhesions, often found in both simple and squamous epithelium. These adhesions are important for ensuring tissue integrity and regulation of paracellular movement of solutes and restricting access of luminal pathogens to underlying tissue compartments. Desmoglein-2 (DSG2) has been shown to regulate numerous cellular processes, including proliferation and apoptosis. Intracellular fragments of DSG2 promote apoptosis in colonic epithelial cells. Mutations in this gene is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC).
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | AH12.2, monoclonal |
| 分子量 | antigen ~150 kDa |
| species reactivity | human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells western blot: 0.5-1.0 μg/mL using HeLa total cell extracts |
| 同位素/亚型 | IgG1 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
