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Anti-eIF-4E

品牌
Sigma-Aldrich
货号
ABE2695
规格纯度
from rabbit, purified by affinity chromatography
参考价格
1415.25 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti-eIF-4E
Eukaryotic translation initiation factor 4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein
产品介绍:

产品说明

一般描述

Eukaryotic translation initiation factor 4E (UniProt: P06730; also known as eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein) is encoded by the EIF4E (also known as EIF4EL1, EIF4F) gene (Gene ID: 1977) in human. elF-4E is a cytoplasmic factor that recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. It is a component of the CYFIP1-EIF4E-FMR1 complex that binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex. Three isoforms of elf-4E have been described that are generated by alternative splicing. Elf-4E contains four 7-methylguanosine-containing mRNA cap binding regions and three EIF4EBP1/2/3 binding regions. Hypophosphorylated EIF4EBP1, EIF4EBP2, and EIF4EBP3 are shown to compete with EIF4G1/EIF4G3 to interact with elF-4E. Phosphorylation of ElF4EBP1 by insulin-stimulated MAP kinase (MAPK1 and MAPK3) cause dissociation of the complex and allows EIF4G1/EIF4G3 to bind and result in initiation of translation. Mutations in ElF4E gene have been linked to autism with impairments in social interaction and communication and developmental abnormalities.

特异性

This rabbit polyclonal antibody detects Eukaryotic translation initiation factor 4E in Human, Mouse, and Rat cells. it targets an epitope with in 14 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Eukaryotic translation initiation factor 4E.

Epitope: unknown

应用

Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 0.9 µg/mL from a representative lot detected eIF-4E in 10 µg of PC12 cell lysate.

Immunocytochemistry Analysis: A 1:100-500 dilution from a representative lot detected eIF-4E in HeLa, A431, HUVEC, and NIH/3T3 cell lines.

Anti-eIF-4E, Cat. No. ABE2695, is a highly specific rabbit polyclonal antibody that targets Eukaryotic translation initiation factor 4E and has been tested in Immunocytochemistry and Western Blotting.

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected eIF-4E in 10 µg of Jurkat cell lysate.

目标描述

~31 kDa observed; 25.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human, rat
species reactivity (predicted by homology)monkey (based on 100% sequence homology)
包装antibody small pack of 25 μg
technique(s)immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG
NCBI登记号NP_001124150
UniProt登记号P06730
运输ambient
Gene Informationhuman ... EIF4E(1977)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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